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KMID : 0381120080300040373
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Genes and Genomics
2008 Volume.30 No. 4 p.373 ~ p.382
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QTL Mapping of Kernel Oil Content of Chromosome 6 in a High Oil Maize Mutant (Zea mays L.)
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Jing Han
Hongwu Wang
Chen Shaojiang
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Abstract
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An infrequent high oil maize mutant CE03005 (white seed color and high oil content) from ethyl methane sulphonate pollen mutagenesis was investigated in this research. A F2 population derived from CE03005 × B73 was used to analysis the genetic effects influencing kernel oil content and to map kernel oil content QTL. Ears from three generations were harvested and the individual kernel oil content of each generation was measured with nuclear magnetic resonance. Genetic analysis showed that the average oil content of white kernels was significantly higher than that of yellow kernels. Genotypic and phenotypic analyses were compared using composite interval mapping. A QTL (oilc6-m1）associated with high kernel oil content was detected in the three generations at bin 6.01 of chromosome 6 near the phenotypic marker SC (seed color). The mapping results were congruent with the genetic analysis. The results explained the reason why the oil content of the white colored kernels was higher than that of the yellow ones because the oil content QTL linked tightly with the seed color gene in chromosome 6 in the mutant. The way of QTL mapping was credible. Molecular and phenotypic markers identified could be used for breeding purpose and would lend convenience in cloning QTL.
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KEYWORD
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high oil maize mutant, kernel oil content, phenotypic marker, quantitative trait loci (QTL), SSR markers
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